Monoclonals

Rural Technologies, Inc. offers an extensive range of monoclonal antibody products. Our monoclonal antibodies are produced as mouse ascites fluid or hybridoma cell culture supernatant. Our products are used for various research applications including (but not limited to): immunohistochemistry, immunofluorescence, and enzyme linked immunosobent assays. We have
exclusive and non-exclusive licensing agreements with a number of universities and guarantee the quality of our products.

Click on image for enlarged view of project locations.

Porcine Reproductive & Respiratory Syndrome Virus (PRRSV)
Monoclonal Antibody: (SR30)
SR30 reacts with all North American and European strains of Porcine
Reproductive and Respiratory Syndrome Virus (PRRSV). This monoclonal
antibody recognizes a highly conserved epitope on the nucleocapsid protein
of PRRSV. The antibody is produced as mouse ascites fluid. Three SR30
products are available: SR30-A (unconjugated ascites fluid); SR30-F (ascites
fluid conjugated with fluorescein isothiocyanate (FITC)); and SR30-B
(ascites conjugated with biotin).

Uses of SR30
SR30-A has been used in immunohistochemistry, indirect fluorescent antibody,
and enzyme linked-immunosorbent assays in research applications. SR30-B has
been used in enzyme linked-immunosorbent assays for antigen detection and
can also be used in competitive ELISAs for antibody detection in research
applications. SR30-F has been used in immunofluorescence tests in research
applications.

Cost
SR30-A (1 mL) $385
SR30-F (1 mL) $385
SR30-B (1 mL) $610
SR30-B(100 uL) $70

Isotype-IgG1
Uses and Recommended Dilutions
SR30-A: IFA - 1:50,000
SR30-A: IHC - 1:10,000
SR30-F: IFA - 1:100

Porcine Reproductive & Respiratory Syndrome Virus(PRRSV)
Monoclonal Antibody: (SDOW17)
SDOW17 reacts with >99% of all North American and European isolates of
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). This monoclonal
antibody recognizes a highly conserved epitope on the nucleocapsid protein
of PRRSV. SDOW17 does not react with the Prime Pac PRRSV vaccine strain.
This antibody is produced as mouse ascites fluid. Three SDOW17 products are
available: SDOW17-A (unconjugated ascites fluid); SDOW17-F (ascites fluid
conjugated with fluorescein isothiocyanate (FITC); and SDOW17-B (ascites
conjugated with biotin).

Uses of SDOW17
SDOW17-A has been used in immunohistochemistry, indirect fluorescent
antibody, and enzyme linked-immunosorbent assays in research applications.
SDOW17-F has been used in immunofluorescence tests in research applications.
SDOW17-B has been used in enzyme linked-immunosorbent assays for antigen
detection in research applications.

Cost
SDOW17-A (1 mL) $385Isotype-IgG1
SDOW17-F (1 mL) $385
SDOW17-B (1 mL) $610
SDOW17-B(100 uL) $70

Isotype-IgG1
Uses and Recommended Dilutions
SDOW17-A: IFA - 1:50,000
SDOW17-A: IHC - 1:10,000
SDOW17-F: IFA - 1:100

Bovine Coronavirus (BCV)
Monoclonal Antibody: (BC 6-4)
BC 6-4 reacts with North American strains of Bovine Coronavirus (BCV). This
monoclonal antibody recognizes an epitope on the nucleocapsid protein of
BCV. The antibody is produced as mouse ascites fluid. One BC 6-4 product is
available: BC 6-4-A (unconjugated ascites fluid).

Uses of BC 6-4
BC 6-4-A has been used for immunohistochemistry, indirect fluorescent
antibody and enzyme linked-immunosorbent assays in research applications.

Cost
BC 6-4-A (1 mL) $385

Isotype-IgG1
Uses and Recommended Dilutions
BC 6-4-A: IFA
BC 6-4-A: IHC
BC 6-4-A: ELISA

Bovine Coronavirus (BCV)
Monoclonal Antibody: (BCV 8F2)
BCV 8F2 reacts with Antigenic Group II viruses from the family
Coronaviridae. This monoclonal antibody recognizes nucleoproteins from both
bovine and elk coronavirus. The antibody is produced in hybridoma cells.

Uses of BCV 8F2
BCV 8F2 has been used in immunohistochemistry assays to detect the presence
of the coronavirus nucleoprotein in formalin fixed tissues.

Click here for more information

Cost
BCV 8F2 (1 mL) $500

Isotype-IgG1
Uses and Recommended Dilutions
BC-8F2-C: IHC - 1:100-1:200

Rotavirus (RV)
Monoclonal Antibody: (RV 11-2)
RV 11-2 reacts with Group A strains of rotavirus (RV). This monoclonal
antibody recognizes an epitope on the VP6 protein of RV. The antibody is
produced as mouse ascites fluid. Three RV 11-2 products are available: RV
11-2-A (unconjugated ascites fluid); RV 11-2-F (ascites fluid conjugated
with fluorescien isothiocyanate (FITC)); and RV 11-2-B (ascites conjugated
with biotin) which is available on request.

Uses of RV 11-2
RV 11-2-A has been used for indirect fluorescent antibody and enzyme
linked-immunosorbent assays in research applications. RV 11-2-B has been
used for antigen detection assays in research applications. RV 11-2-F has
been used for immunofluorescence tests in research applications.

Cost
RV 11-2-A (1 mL) $385
RV 11-2-B (1 mL) $385
RV 11-2-F (1 mL) $385

Isotype-IgG2a
Uses and Recommended Dilutions
RV 11-2-A: IFA
RV 11-2-A: ELISA
RV 11-2-F: IFA - 1:100
Rv 11-2-B: 1:500

E. coli
Monoclonal Antibody: EC 4A3, EC F18, and EC K88
EC 4A3 reacts with Escherichia coli (E. coli) strains that express the 987P
pilus antigen. The antibody is produced as mouse ascites fluid. One EC 4A3
product is available: EC 4A3-A (unconjugated ascites fluid).

EC F18 has been used for indirect fluorescent antibody assays to detect the
presence of the F18 fimbrial antigen in research applications.

EC K88 The anti-K88ac monoclonal antibody was created against the K88
fimbria of enterotoxigenic Escherichia coli (ETEC). The antibody reacts with
K88 fimbria of ETEC and blocks the binding of K88 + bacteria to intestinal
epithelial cells. The antibody is produced in hybridoma cells and is
available as cell culture supernatant.

Cost
EC 4A3-A (1 mL) $385
EC F18-C (5 mL) $385

Isotype-IgG1
Uses and Recommended Dilutions
EC 4A3-A: IFA - 1:1000
EC F18-C: IFA - 1:500

Porcine Circovirus (PCV)
Monoclonal Antibody: (PCV2)
The PCV2 monoclonal antibody reacts with antigenic group II viruses from the porcine circovirus family. This monoclonal antibody was found to react by a dot-immunoblot assay to purified virus; however, the protein specificity has not yet been determined. The antibody is produced as mouse ascites fluid.

Uses of PCV2
PCV2 has been used for indirect flourescent antibody assays (1:1000) and immunohistochemistry (1:500). Upon receipt, the frozen ascites should be thawed, dispensed in small aliquots and frozen at -20C. If multiple aliquots are withdrawn from the stock, sterile techniques should be practiced to prevent contamination of the ascites fluid. Individual laboratories and investigators are advised to determine the optimal working dilution for their protocol.

Cost
PCV2 (1 mL) $385

Isotype-IgG2
Uses and Recommended Dilutions
PCV2-A: IFA - 1:1000
PCV2-A: IHC - 1:500

Anti-ILT Monoclonal Antibody: (ILT-004)
The anti-ILT monoclonal antibody provides nuclear staining of trachael cells infected with the infectious laryngotracheitis virus (ILTV) antigen. The antibody is produced in hybridoma cells and is available as cell culture supernatant.

Uses of ILT-004
ILT-004 has been used for indirect flourescent antibody assays to detect the presence of the infectious laryngotracheitis virus (ILTV) antigen in research applications.

Cost
ILT-004 (5 mL) $385

Isotype-IgG2b
Uses and Recommended Dilutions
ILT-004: IFA - 1:50